Crystal structure determination of hyaluronidase, a major bee venom allergen, in complex with an IgG Fab fragment and purification and biophysical characterization of bovine testes hyaluronidase
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گزش‌های متعدد زنبور عسل: گزارش چهار مورد
Background: Insect stings can cause local or systemic reactions that range from mild to fatal, and are among the most common causes of anaphylaxis. The major allergens of honeybee venom are phospholipase A2, hyaluronidase, acid phosphatase, allergen C and melitin. Phospholipase and melitin induce hemolysis, rhabdomyolysis and liver damage due to cell membrane breakdown, damage of the vascular e...
متن کاملStudy of the Effects of Gamma Irradiation on Immunological and Pathological Characteristics of Bee Venom in a Mouse Animal Model
Extended Abstract Introduction and Objective: People are sensitive to bee venom and the presence of allergenic compounds in the venom has made it difficult to use and determine the optimal dose for treatment. The aim of this study was to reduce allergenic compounds and to evaluate the quality of gamma ray processed bee venom. Material and Methods: Venom samples were irradiated at dose...
متن کاملBee venom hyaluronidase is homologous to a membrane protein of mammalian sperm.
The venom of honeybees, Apis mellifera, contains several biologically active peptides and two enzymes, one of which is a hyaluronidase. By using degenerate oligonucleotides derived from the amino-terminal sequence of this hyaluronidase reported by others, clones encoding the precursor for this enzyme could be isolated from a cDNA library prepared from venom glands of worker bees. The deduced am...
متن کاملAllergy to insect stings. II. Phospholipase A: the major allergen in honeybee venom.
In order to determine the proteins of major allergenic importance in honeybee venom (Apis mellifera) it was chromatographed on G-50 Sephadex. The four major protein peaks eluted were identified as hyaluronidase, phospholipase, melittin, and apamin. Testing these preparations on the leukocytes of 6 honeybee-sensitive patients, with the in vitro method of histamine release, revealed that all indi...
متن کاملThe soluble hyaluronidase from bull testes is a fragment of the membrane-bound PH-20 enzyme.
The membrane-bound PH-20 hyaluronidase is known to be essential for fertilization. Here we addressed the question whether the soluble hyaluronidase from bull teste is related to the PH-20 polypeptide. The sequence of the membrane-bound PH-20 hyaluronidase from bovine sperm was determined via cDNA cloning. In parallel, from a commercial preparation of bovine hyaluronidase the major 60-kDa form w...
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تاریخ انتشار 2007